primer pairs for pcna and cyclin d1  (SuperArray Bioscience Corporation)

Journal: Cancer Science

Article Title: Metformin suppresses intestinal polyp growth in Apc Min/+ mice

doi: 10.1111/j.1349-7006.2008.00933.x

Figure Lengend Snippet: Immunohistochemistry for β‐catenin, cell proliferation assay using 5‐bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA), apoptosis assay, and gene expression analyses of cyclin D1 and c‐myc in the tumor cells. (a) HE staining and immunohistochemical staining for β‐catenin, BrdU and PCNA in intestinal tumors from Apc Min/+ mice fed a basal diet or treated with metformin. Transferase deoxytidyl uridine end labeling staining in tumors are also demonstrated. (b,c,d) Calculations for the BrdU, PCNA and apoptotic indices are detailed in Materials and Methods section. No significant difference was observed in each index category between metformin‐treated and control groups. Gene expression of (e) cyclin D1 and (f) c‐myc in intestinal polyps using real time reverse transcription polymerase chain reaction analysis are also shown. β‐Actin was used as the internal control. No significant difference was observed in these gene expression levels between metformin‐treated and control groups. Bars represent standard error.

Article Snippet: Probes and primer pairs specific for cyclin D1 , c‐myc and β‐actin were purchased from Applied Biosystems.

Techniques: Immunohistochemistry, Proliferation Assay, Apoptosis Assay, Expressing, Staining, Immunohistochemical staining, End Labeling, Reverse Transcription, Polymerase Chain Reaction